Transmission
Neuromuscular transmission involves the events leading from the liberation of acetylcholine (ACh) at
the motor nerve terminal to the generation of end plate currents (EPCs) at the postjunctional site. Release of
ACh is initiated by membrane depolarization and influx of Ca++at the nerve terminal.This leads to a
complex process involving docking and fusion of synaptic vesicles with active sites at the presynaptic mem-
brane. Because ACh is released by exocytosis, functional transmitter release takes place in a quantal
fashion. Each quantum corresponds to the contents of one synaptic vesicle (about 10,000 ACh molecules), and about 200 quanta are released with each nerve action potential.
ACh diffusing across the synaptic cleft may bind to ACh receptors (AChRs) to produce an electrical re-
sponse, interact with acetylcholinesterase (AChE) and be hydrolyzed, or diffuse into the systemic circulation.
AChRs are located primarily at the peaks of the subsynaptic folds, whereas AChE is distributed uniformly in
the basal lamina at the subsynaptic membrane .The AChR consists of five subunits surrounding an
ion-conducting channel . Activation of the binding sites on the two alpha-subunits results in a conformational change.This allows the simultaneous inflow of Na+ and Ca ++and outflow of K+, with a net inflow of
positive charge. The response to a spontaneously secreted quantum of ACh (that is, activation of several
thousand AChRs) is seen as a miniature EPC. With nerve stimulation, many quanta are released synchronously to produce a full-sized EPC, which is the summated response of the 200 or so individual miniature EPCs.The EPC is a local graded current that in normal conditions triggers an action potential in the adjacent muscle membrane
Drugs may modify transmission by affecting either the release of transmitter or the interaction of ACh with
its receptor. An increase in transmitter release is produced by substances that induce repetitive firing in the
motor nerve, prolong the nerve action potential, or promote Ca++influx at the nerve terminal.An increase in
the postjunctional response is produced by drugs that inhibit AChE (and thereby increase the synaptic con-
centration of ACh), whereas a decrease in response is produced by drugs that block the binding sites or ion-
conducting channel of the AChR.
Changes in miniature EPC frequency, amplitude,and duration can be used to identify the sites of drug
action. In general, a change in frequency indicates a prejunctional action, while a change in amplitude reflects a postjunctional effect. Drugs that inhibit AChE give rise to a larger and more prolonged miniature
EPC, whereas those that block the AChR binding site produce a decrease in miniature EPC amplitude.
Agents that obstruct the open channel of the AChR cause a reduction in miniature EPC amplitude and duration.
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Unknown - Tuesday 21 December 2010
